Journal: Med (New York, N.y.)

Article Title: Twin study dissects CXCR3 + memory B cells as non-heritable feature in multiple sclerosis

doi: 10.1016/j.medj.2024.02.013

Figure Lengend Snippet: Twin study identifies CXCR3 + memory B cells as non-heritable component in multiple sclerosis Mass cytometry analysis of the peripheral B cell compartment of 57 twin pairs discordant for multiple sclerosis (MS). Uniform manifold approximation and projection (UMAP) of 100 cells/sample randomly drawn from the combined dataset (A, left). Colors indicate FlowSOM clustering and concomitant manual annotation, and heatmap displays corresponding expression profiles (A, right). UMAP overlay showing the CXCR3 expression across B cells (B). Violin plots showing the frequency of B cell clusters among total B cells for twins with MS and non-MS twins across all twin pairs analyzed (n = 57; C) and twin pairs in which the twin with MS did not receive immunomodulatory treatment (n = 20; D). Dashed line indicates twinship. Volcano plot showing the differentially expressed genes between CXCR3 + and CXCR3 − memory B cells in CITE-seq data of patients with MS (n = 20; E). Dot plot showing gene expression of indicated B cell signature genes across B cell clusters in the CITE-seq dataset (n = 20; F). Schematic (left), representative biaxial flow cytometry plots (middle), and violin plot (right) showing CXCR3 + and CXCR3 − memory B cells derived from healthy blood donors differentiated into antibody-secreting cells in the presence of CD40L and interleukin-21 (IL-21) (n = 8; G). Violin plots showing the CXCL10 concentrations in the serum and cerebrospinal fluid of patients with newly diagnosed MS (n = 11 pairs; H). Violin plots showing the inter-twin-pair difference for the frequency of CXCR3 + memory B cells separated by treatment of the MS-affected twin. ALZ, alemtuzumab (n = 3); DMF, dimethyl fumarate (n = 2); FTY, fingolimod (n = 4); GLAT, glatiramer acetate (n = 8); IFN, type 1 interferons (n = 14); NAT, natalizumab (n = 4); TFM, teriflunomide (n = 2; I). Effect size for the intra-twin-pair changes in B cell subset frequency of natalizumab-treated twin pairs (n = 4) compared to twin pairs in which the MS-affected twin did not receive immunomodulatory treatment (n = 20; J). Comparisons were performed using a two-sided paired non-parametric Wilcoxon signed-rank test (C, D, G, and H) or a two-sided unpaired non-parametric Mann-Whitney-Wilcoxon test (I and J). Differential gene expression was computed using a two-sided unpaired non-parametric Mann-Whitney-Wilcoxon test with Benjamini-Hochberg adjustment to control false discovery.

Article Snippet: Mass cytometry data of twin pairs discordant for MS , Ingelfinger et al. , Mendeley Data: https://doi.org/10.17632/fzs5ph5p8s.1.

Techniques: Mass Cytometry, Expressing, Gene Expression, Flow Cytometry, Derivative Assay, MANN-WHITNEY, Control

Journal: Med (New York, N.y.)

Article Title: Twin study dissects CXCR3 + memory B cells as non-heritable feature in multiple sclerosis

doi: 10.1016/j.medj.2024.02.013

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Article Snippet: Mass cytometry data of twin pairs discordant for MS , Ingelfinger et al. , Mendeley Data: https://doi.org/10.17632/fzs5ph5p8s.1.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Mass Cytometry, Expressing, Software, Flow Cytometry

Journal: Cell reports

Article Title: Multimodal analysis of dysregulated heme metabolism, hypoxic signaling, and stress erythropoiesis in Down syndrome

doi: 10.1016/j.celrep.2024.114599

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Article Snippet: HTP mass cytometry data , Galbraith et al. , Flow Repository: FR-FCM-Z5GE, Synapse: https://doi.org/10.7303/syn31488783.

Techniques: Binding Assay, Isolation, Biomarker Discovery, Staining, Antibody Labeling, Clinical Proteomics, Mass Cytometry, Software, Sequencing